SsoAdvanced™ universal SYBR®Green supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein,Sso7d, stabilizes the polymerase-template complex, providingsuperior inhibitor tolerance, increasedprocessivity, specificity, and greater speed without affecting PCRsensitivity, efficiency, or reproducibility.

SsoAdvanced™ universal SYBR®Green supermix lets you:

• Use any real-time PCR system— the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
• Achieve superior real-time PCR results under various conditions— our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
• Increase your qPCR sensitivity and efficiency of detection from compromised samples— the Sso7d fusion polymerase hasincreased resistanceto a wide variety of PCR inhibitors, which helps providebetter sensitivityand overall performance
• Decrease time to results without compromising qPCR data quality— the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
• Obtain better results with Bio-Rad'sPrimePCR™ assays— real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance

Applications and Uses of SsoAdvanced™ Universal SYBR®Green Supermix

• qPCR/real-time PCR
• Gene expression analysis
• Pathway analysis
• Absolute quantification
• Chromatin immunoprecipitation (ChIP) qPCR
• Mutation detection
• Pathogen detection
• Viral detection (load)
• Characterization of genetically modified organisms (GMO)
• Genetic profiling

Packaging Options

Number of 20 μl Reactions	Total Volume	Number of Vials	Catalog #
200	2 ml	2 x 1 ml	#172-5270
500	5 ml	5 x 1 ml	#172-5271
1,000	10 ml	10 x 1 ml	#172-5272
2,500	25 ml	5 x 5 ml	#172-5274
5,000	50 ml	10 x 5 ml	#172-5275

Instrument Compatibility

The SsoAdvanced™ universal SYBR®Green supermix is compatible with all commercially available and allBio-Rad real-time qPCR systems.

Related Products

We have developedSsoAdvanced universal probes supermix, which is available in several formats (200–5,000 reactions).

Bio-Rad offers a complete workflow solution for real-time PCR, including:

• RNA isolation kits
• Reverse transcription kits
• Real-time PCR supermixes

More Information

To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-stepdesign and optimization protocols. We also provide insights intoanalysis of qPCR dataandtroubleshooting reactions.

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.