Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.

• For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol
• For tank blotting of native gels, without methanol
• As a running buffer for native gels

Precast gels that can be used for native electrophoresis

Precast Gel Type	Size	Gel Chemistry
Mini-PROTEAN®TGX™	Mini	Laemmli-like, long shelf life, fast separation with high resolution
Criterion™ TGX™	Midi
Mini-PROTEAN®TGX Stain-Free™	Mini	Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection
Criterion™ TGX Stain-Free™	Midi
Criterion™ Tris-HCl	Midi	Standard Laemmli
Criterion Stain Free™ Tris-HCl	Midi	Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection
Criterion XT Tris-Acetate	Midi	Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins

Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) of interest are being separated, change the polarity of the electrodes, since they have a net positive charge.

Packaging Options

10x Buffer Volume	Catalog Number
1 L	1610734
5 L cube	1610771

Related Products

• PrecisionAb™ Validated Western Blotting Antibodies
• Native Sample Buffer (1610738)
• Criterion Staining/Blotting Trays with lids (3459921)
• 10x Phosphate Buffered Saline (PBS) (1610780)
• 10x Tris Buffered Saline (TBS) (1706435)
• 1x Phosphate Buffered Saline (PBS) with 1% Casein (1610783)
• 1x Tris Buffered Saline (TBS) with 1% Casein (1610782)
• Blotting-Grade Blocker, nonfat dry milk (1706404)

Related Categories

• Protein Gels
• Western Blotting Membranes and Filter Paper
• Semi-Dry and Rapid Blotting Systems
• Wet/Tank Blotting Systems
• Power Supplies
• Transfer Buffers
• Immunodetection Reagents and Kits
• Gel and Western Imaging Systems

Supporting Documents

• Mini-PROTEAN Precast GelsInstruction Manual and Application Guide
• Criterion Gel Application GuideInstruction Manual
• Mini Trans-Blot®CellInstruction Manual
• Trans-Blot SD CellQuick Reference Guide
• Trans-Blot SD Semi-Dry Transfer CellInstruction Manual

Online Applications

• Protein Electrophoresis
• Western Blotting
• Imaging and Analysis